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STEMCELL Technologies Inc brainphys neuronal medium 05790
Brainphys Neuronal Medium 05790, supplied by STEMCELL Technologies Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/brainphys+neuronal+medium+05790/brainphys+neuronal+medium+st+05793/bio_rxiv__2025__07__15__664947-64-17-20
Average 90 stars, based on 1 article reviews
brainphys neuronal medium 05790 - by Bioz Stars, 2026-09
90/100 stars

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Related Articles

Cell Culture:

Article Title: Glioma-derived SPARCL1 promotes the formation of peritumoral neuron–glioma synapses
Article Snippet: .. Neurons were cultured in BrainPhys neuronal medium (StemCell Technologies, 05790, Canada), supplemented with GlutaMAX (Procell, PB180419 , China), 1% penicillin/streptomycin (Gibco, 15140-122, USA), B27 supplement (Invitrogen, 17504-044, USA), BDNF (10 ng ml − 1 ; PeproTech, 450-02, USA), GDNF (5 ng ml − 1 ; PeproTech, 450 − 10, USA), TRO19622 (5 μm; Tocris, T3077, UK), β-mercaptoethanol (Gibco, 21985023, USA), and 2% fetal bovine serum (Gibco, 10099141 C, USA). ..

Article Title: Glioma-derived SPARCL1 promotes the formation of peritumoral neuron-glioma synapses.
Article Snippet: [1].. Gliomas infiltrate the brain parenchyma diffusely, and patients often present with symptoms such as headache, seizures, cognitive impairment and focal neurological deficits [2], all of which severely affect their quality of life.. Gliomas and surrounding peritumoral tissues are universally recognized to contain three distinct types of synapses: synapses formed between glioma cells, synapses formed between neurons, and neuron‒glioma synapses (NGSs).

Article Title: Genotype–Phenotype Distinctions in Spastic Paraplegia 4 Reveal HDAC6 as a Therapeutic Target
Article Snippet: Single-wells containing 60 electrodes (Multi Channel Systems, MEA2100-Systems), were coated with Poly-L-Ornithine (Sigma Aldrich, P4957) overnight at room temperature then washed 3-5 times with sterile ddH 2 O. .. The wells were fully dried, and organoids were placed centrally covering all the electrodes and cultured in BrainPhys Neuronal Medium (Stem Cell Technologies, 05790) supplemented with BDNF and NT3. ..

Staining:

Article Title: Glioma-derived SPARCL1 promotes the formation of peritumoral neuron-glioma synapses.
Article Snippet: [1].. Gliomas infiltrate the brain parenchyma diffusely, and patients often present with symptoms such as headache, seizures, cognitive impairment and focal neurological deficits [2], all of which severely affect their quality of life.. Gliomas and surrounding peritumoral tissues are universally recognized to contain three distinct types of synapses: synapses formed between glioma cells, synapses formed between neurons, and neuron‒glioma synapses (NGSs).

Real-time Polymerase Chain Reaction:

Article Title: Glioma-derived SPARCL1 promotes the formation of peritumoral neuron-glioma synapses.
Article Snippet: [1].. Gliomas infiltrate the brain parenchyma diffusely, and patients often present with symptoms such as headache, seizures, cognitive impairment and focal neurological deficits [2], all of which severely affect their quality of life.. Gliomas and surrounding peritumoral tissues are universally recognized to contain three distinct types of synapses: synapses formed between glioma cells, synapses formed between neurons, and neuron‒glioma synapses (NGSs).

Synthesized:

Article Title: Glioma-derived SPARCL1 promotes the formation of peritumoral neuron-glioma synapses.
Article Snippet: [1].. Gliomas infiltrate the brain parenchyma diffusely, and patients often present with symptoms such as headache, seizures, cognitive impairment and focal neurological deficits [2], all of which severely affect their quality of life.. Gliomas and surrounding peritumoral tissues are universally recognized to contain three distinct types of synapses: synapses formed between glioma cells, synapses formed between neurons, and neuron‒glioma synapses (NGSs).

other:

Article Title: Directed differentiation of human hindbrain neuroepithelial stem cells recapitulates cerebellar granule neurogenesis.
Article Snippet: On day 7, differentiating cells were confluent again and split 1:3-1:4 into Step 2 differentiation medium: BrainPhys Neuronal Medium (STEMCELL Technologies, 05790), 1× N2, 0.01× B27, 2.5 ng/ml BMP6, 5 ng/ml BMP7, 50 ng/ml BMP12/GDF7, 5 ng/ml SHH (R&D Systems, 8908-SH), 2 ng/ml JAG1 (1277-JG-050, 1277-JG-050), 10 ng/ml NT3 (R&D Systems, 267-N3-025/CF) and 100 ng/ ml BDNF (R&D Systems, 248-BDB-01 M/CF).

Article Title: In vitro modeling of the human dopaminergic system using spatially arranged ventral midbrain–striatum–cortex assembloids
Article Snippet: BrainPhys Neuronal Medium (Stemcell Technologies, cat. no. 05790), 2% B27+A, 1% N2 Supplement, 1 ml CD Lipid Concentrate (Thermo Fisher Scientific, cat. no.11905031), 1% Antibiotic-Antimycotic, 1:147 20% glucose solution, 20 ng ml −1 BDNF (Stemcell Technologies, cat. no. 78005.3), 20 ng ml −1 GDNF (Stemcell Technologies, cat. no. 78057.3), 1 mM db-cAMP (Santa Cruz Biotechnology, cat. no. sc-201567C).

Article Title: Reconstitution of Human Brain Cell Diversity in Organoids via Four Protocols
Article Snippet: BrainPhys Neuronal Medium (Stemcell Technologies, cat. no. 05790), 2% B27+A, 1% N2 Supplement, 1% CD Lipid Concentrate (Thermo Fisher Scientific, cat. no.11905031), 1% Antibiotic-Antimycotic, 1:147 20% glucose solution.

Imaging:

Article Title: Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes
Article Snippet: Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930, StemCell Technologies). .. For arrayed imaging experiments, cells were thawed, seeded into 384 well plates coated with poly-l-ornithine (P4957, Sigma-Aldrich) and Laminin-511(RL511-175, Peprotech), and maintained until experimental endpoint in hNIL Maturation media, containing: BrainPhys Neuronal Medium (05790, StemCell Technologies), MEM Nonessential Amino Acids (1X, 11140050, ThermoFisher), GlutaMAX(1X, 35050061, ThermoFisher), B-27 Plus Supplement (1X, A3582801, ThermoFisher), Recombinant Human BDNF Protein (1ug/ml, 248-BDB-MTO, R&D Systems), Neurotrophin-3 (1ug/ml, 267-N3-025, R&D Systems), EdU (5-ethynyl-2’-deoxyuridine, A10044, ThermoFisher) and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). ..

Recombinant:

Article Title: Deep Learning Analysis on Images of iPSC-derived Motor Neurons Carrying fALS-genetics Reveals Disease-Relevant Phenotypes
Article Snippet: Progenitor cells were cryopreserved for future use in micronic vials in CryoStorCS10 (07930, StemCell Technologies). .. For arrayed imaging experiments, cells were thawed, seeded into 384 well plates coated with poly-l-ornithine (P4957, Sigma-Aldrich) and Laminin-511(RL511-175, Peprotech), and maintained until experimental endpoint in hNIL Maturation media, containing: BrainPhys Neuronal Medium (05790, StemCell Technologies), MEM Nonessential Amino Acids (1X, 11140050, ThermoFisher), GlutaMAX(1X, 35050061, ThermoFisher), B-27 Plus Supplement (1X, A3582801, ThermoFisher), Recombinant Human BDNF Protein (1ug/ml, 248-BDB-MTO, R&D Systems), Neurotrophin-3 (1ug/ml, 267-N3-025, R&D Systems), EdU (5-ethynyl-2’-deoxyuridine, A10044, ThermoFisher) and Doxycycline Hydrochloride (1ug/ml, 631311, Clontech). ..



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Image Search Results


Journal: iScience

Article Title: Fate plasticity of interneuron specification

doi: 10.1016/j.isci.2025.112295

Figure Lengend Snippet:

Article Snippet: From Day 70, organoids were cultured in neuronal maturation media containing BrainPhys Neuronal Medium (Stem Cell Technologies # 05790), 10% (v/v) Hyclone characterized fetal bovine serum, 1X N-2 Supplement, 1X Chemically Defined Lipid Concentrate (Thermo Fisher Scientific # 11905031), 1X B-27 Supplement (Thermo Fisher Scientific # 17504044), 100 U/mL Penicillin/Streptomycin, and 1% v/v Matrigel GFR.

Techniques: Virus, Plasmid Preparation, Recombinant, Membrane, Imaging, RNA Sequencing, Software, Cell Culture, Modification, Knock-Out, Passaging